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Riboswitch (T-box)-mediated control of tRNA-dependent amidation in Clostridium acetobutylicum rationalizes gene and pathway redundancy for asparagine and asparaginyl-trnaasn synthesis.
Riboswitch (T-box)-mediated control of tRNA-dependent amidation in Clostridium acetobutylicum rationalizes gene and pathway redundancy for asparagine and asparaginyl-trnaasn synthesis.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2012 Jun 08; Vol. 287 (24), pp. 20382-94. Date of Electronic Publication: 2012 Apr 13. - Publication Year :
- 2012
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Abstract
- Analysis of the Gram-positive Clostridium acetobutylicum genome reveals an inexplicable level of redundancy for the genes putatively involved in asparagine (Asn) and Asn-tRNA(Asn) synthesis. Besides a duplicated set of gatCAB tRNA-dependent amidotransferase genes, there is a triplication of aspartyl-tRNA synthetase genes and a duplication of asparagine synthetase B genes. This genomic landscape leads to the suspicion of the incoherent simultaneous use of the direct and indirect pathways of Asn and Asn-tRNA(Asn) formation. Through a combination of biochemical and genetic approaches, we show that C. acetobutylicum forms Asn and Asn-tRNA(Asn) by tRNA-dependent amidation. We demonstrate that an entire transamidation pathway composed of aspartyl-tRNA synthetase and one set of GatCAB genes is organized as an operon under the control of a tRNA(Asn)-dependent T-box riboswitch. Finally, our results suggest that this exceptional gene redundancy might be interconnected to control tRNA-dependent Asn synthesis, which in turn might be involved in controlling the metabolic switch from acidogenesis to solventogenesis in C. acetobutylicum.
- Subjects :
- Asparagine genetics
Aspartate-Ammonia Ligase genetics
Bacterial Proteins genetics
Clostridium acetobutylicum genetics
RNA, Bacterial genetics
RNA, Transfer, Amino Acyl genetics
Asparagine biosynthesis
Aspartate-Ammonia Ligase biosynthesis
Bacterial Proteins biosynthesis
Clostridium acetobutylicum metabolism
RNA, Bacterial metabolism
RNA, Transfer, Amino Acyl biosynthesis
Riboswitch physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 287
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22505715
- Full Text :
- https://doi.org/10.1074/jbc.M111.332304