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Panax ginseng modulates cytokines in bone marrow toxicity and myelopoiesis: ginsenoside Rg1 partially supports myelopoiesis.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (4), pp. e33733. Date of Electronic Publication: 2012 Apr 16. - Publication Year :
- 2012
-
Abstract
- In this study, we have demonstrated that Korean Panax ginseng (KG) significantly enhances myelopoiesis in vitro and reconstitutes bone marrow after 5-flurouracil-induced (5FU) myelosuppression in mice. KG promoted total white blood cell, lymphocyte, neutrophil and platelet counts and improved body weight, spleen weight, and thymus weight. The number of CFU-GM in bone marrow cells of mice and serum levels of IL-3 and GM-CSF were significantly improved after KG treatment. KG induced significant c-Kit, SCF and IL-1 mRNA expression in spleen. Moreover, treatment with KG led to marked improvements in 5FU-induced histopathological changes in bone marrow and spleen, and partial suppression of thymus damage. The levels of IL-3 and GM-CSF in cultured bone marrow cells after 24 h stimulation with KG were considerably increased. The mechanism underlying promotion of myelopoiesis by KG was assessed by monitoring gene expression at two time-points of 4 and 8 h. Treatment with Rg1 (0.5, 1 and 1.5 µmol) specifically enhanced c-Kit, IL-6 and TNF-α mRNA expression in cultured bone marrow cells. Our results collectively suggest that the anti-myelotoxicity activity and promotion of myelopoiesis by KG are mediated through cytokines. Moreover, the ginsenoside, Rg1, supports the role of KG in myelopoiesis to some extent.
- Subjects :
- Animals
Bone Marrow drug effects
Bone Marrow pathology
Bone Marrow Cells metabolism
Cells, Cultured
Fluorouracil antagonists & inhibitors
Granulocyte-Macrophage Colony-Stimulating Factor metabolism
Hematocrit
Interleukin-3 metabolism
Interleukin-6 biosynthesis
Male
Mice
Mice, Inbred C57BL
Panax metabolism
Proto-Oncogene Proteins c-kit biosynthesis
Spleen drug effects
Spleen pathology
Thymus Gland drug effects
Thymus Gland pathology
Tumor Necrosis Factor-alpha biosynthesis
Bone Marrow metabolism
Ginsenosides pharmacology
Myelopoiesis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22523542
- Full Text :
- https://doi.org/10.1371/journal.pone.0033733