Back to Search
Start Over
Peripheral blood stem cell transplant-related Plasmodium falciparum infection in a patient with sickle cell disease.
- Source :
-
Transfusion [Transfusion] 2012 Dec; Vol. 52 (12), pp. 2677-82. Date of Electronic Publication: 2012 Apr 27. - Publication Year :
- 2012
-
Abstract
- Background: Although transmission of Plasmodium falciparum (Pf) infection during red blood cell (RBC) transfusion from an infected donor has been well documented, malaria parasites are not known to infect hematopoietic stem cells. We report a case of Pf infection in a patient 11 days after peripheral blood stem cell transplant for sickle cell disease.<br />Study Design and Methods: Malaria parasites were detected in thick blood smears by Giemsa staining. Pf HRP2 antigen was measured by enzyme-linked immunosorbent assay on whole blood and plasma. Pf DNA was detected in whole blood and stem cell retention samples by real-time polymerase chain reaction using Pf species-specific primers and probes. Genotyping of eight Pf microsatellites was performed on genomic DNA extracted from whole blood.<br />Results: Pf was not detected by molecular, serologic, or parasitologic means in samples from the recipient until Day 11 posttransplant, coincident with the onset of symptoms. In contrast, Pf antigen was retrospectively detected in stored plasma collected 3 months before transplant from the asymptomatic donor. Pf DNA was detected in whole blood from both the donor and the recipient after transplant, and genotyping confirmed shared markers between donor and recipient Pf strains. Lookback analysis of RBC donors was negative for Pf infection.<br />Conclusions: These findings are consistent with transmission by the stem cell product and have profound implications with respect to the screening of potential stem cell donors and recipients from malaria-endemic regions.<br /> (© 2012 American Association of Blood Banks.)
- Subjects :
- Adult
Anemia, Sickle Cell blood
DNA, Protozoan genetics
Emigrants and Immigrants
Female
Genotype
Humans
Malaria, Falciparum blood
Plasmodium falciparum genetics
Sierra Leone ethnology
United States
Anemia, Sickle Cell therapy
Hematopoietic Stem Cell Transplantation adverse effects
Malaria, Falciparum transmission
Plasmodium falciparum isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1537-2995
- Volume :
- 52
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 22536941
- Full Text :
- https://doi.org/10.1111/j.1537-2995.2012.03673.x