Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory clinical specimens using a rapid real-time polymerase chain reaction assay.

Authors :: Diaz MH
Winchell JM
Source :: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2012 Jul; Vol. 73 (3), pp. 278-80. Date of Electronic Publication: 2012 Apr 25.
Publication Year :: 2012
Abstract: We developed a rapid real-time polymerase chain reaction assay for detecting Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory specimens. This procedure provides over 5 times faster results compared to existing methods while maintaining equivalent detection rates for specimens containing limited target organisms.<br /> (Published by Elsevier Inc.)

Subjects :: Chlamydophila Infections diagnosis
Chlamydophila pneumoniae genetics
Humans
Molecular Diagnostic Techniques methods
Mycoplasma pneumoniae genetics
Pneumonia, Mycoplasma diagnosis
Sensitivity and Specificity
Time Factors
Bacteriological Techniques methods
Chlamydophila Infections microbiology
Chlamydophila pneumoniae isolation & purification
Exudates and Transudates microbiology
Mycoplasma pneumoniae isolation & purification
Pneumonia, Mycoplasma microbiology
Real-Time Polymerase Chain Reaction methods

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