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Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging.
- Source :
-
Free radical research [Free Radic Res] 2012 Sep; Vol. 46 (9), pp. 1093-8. Date of Electronic Publication: 2012 May 21. - Publication Year :
- 2012
-
Abstract
- The levels of the oxidised forms of guanosine in leukocytes, plasma and urine of Macaca mulatta were determined using a sensitive method based on high-performance liquid chromatography-triple quadruple mass spectrometry (LC-MS/MS). The amounts of 8-oxo-7,8-dihydrodeoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosin (8-oxoGsn), derived from DNA and RNA, respectively, increased with age in leukocytes. The measurement of the free forms of oxidised guanosine revealed similar age-dependent increases of 8-oxo-dGsn and 8-oxoGsn in both plasma and urine, which showed considerably larger amounts of 8-oxoGsn than 8-oxo-dGsn. The 8-oxoGsn content of urine could be a useful biomarker for evaluating aging, as age-dependent increases of 8-oxoGsn are more evident in urine compared to plasma and because urine samples are readily available.
- Subjects :
- Aging metabolism
Animals
Biomarkers analysis
Biomarkers metabolism
Chromatography, High Pressure Liquid
Female
Guanosine analysis
Guanosine metabolism
Leukocytes chemistry
Macaca mulatta
Tandem Mass Spectrometry
Aging urine
DNA metabolism
Guanosine analogs & derivatives
Leukocytes metabolism
Oxidative Stress
RNA metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1029-2470
- Volume :
- 46
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 22551207
- Full Text :
- https://doi.org/10.3109/10715762.2012.689428