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Immunohistochemistry and detection of proliferating cells by BrdU.

Authors :
Kyryachenko S
Kyrylkova K
Leid M
Kioussi C
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 887, pp. 33-9.
Publication Year :
2012

Abstract

Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in -tissue. The method exploits the principle that the target antigen is recognized by specific antibody and is visualized using different detection systems. The subject of this chapter is simultaneous immunohistochemical detection of protein antigens and proliferation marker BrdU in the developing tooth.

Subjects

Subjects :
Animals
Bromodeoxyuridine
Cell Proliferation
Mice
Tooth embryology
Embryo, Mammalian cytology
Embryo, Mammalian metabolism
Frozen Sections methods
Immunohistochemistry methods

Details

Language :
English
ISSN :
1940-6029
Volume :
887
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
22566044
Full Text :
https://doi.org/10.1007/978-1-61779-860-3_4
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