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BM61 of Bombyx mori nucleopolyhedrovirus: its involvement in the egress of nucleocapsids from the nucleus.
- Source :
-
FEBS letters [FEBS Lett] 2012 Apr 05; Vol. 586 (7), pp. 990-5. Date of Electronic Publication: 2012 Mar 03. - Publication Year :
- 2012
-
Abstract
- All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm.<br /> (Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Line
Cell Nucleus metabolism
Cell Nucleus ultrastructure
Cytoplasm metabolism
Cytoplasm ultrastructure
Gene Deletion
Gene Knockout Techniques
Genes, Reporter
Microscopy, Electron, Transmission
Nuclear Envelope metabolism
Nuclear Envelope ultrastructure
Nuclear Envelope virology
Nucleocapsid ultrastructure
Nucleopolyhedroviruses genetics
Nucleopolyhedroviruses growth & development
Nucleopolyhedroviruses ultrastructure
Occlusion Body Matrix Proteins
Promoter Regions, Genetic
RNA, Messenger metabolism
Real-Time Polymerase Chain Reaction
Recombinant Fusion Proteins metabolism
Recombinant Proteins metabolism
Salivary Glands metabolism
Salivary Glands ultrastructure
Salivary Glands virology
Viral Proteins genetics
Viral Structural Proteins genetics
Viral Structural Proteins metabolism
Bombyx virology
Cell Nucleus virology
Cytoplasm virology
Nucleocapsid metabolism
Nucleopolyhedroviruses metabolism
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 586
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 22569252
- Full Text :
- https://doi.org/10.1016/j.febslet.2011.12.040