Back to Search Start Over

BM61 of Bombyx mori nucleopolyhedrovirus: its involvement in the egress of nucleocapsids from the nucleus.

Authors :
Shen H
Chen K
Source :
FEBS letters [FEBS Lett] 2012 Apr 05; Vol. 586 (7), pp. 990-5. Date of Electronic Publication: 2012 Mar 03.
Publication Year :
2012

Abstract

All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm.<br /> (Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3468
Volume :
586
Issue :
7
Database :
MEDLINE
Journal :
FEBS letters
Publication Type :
Academic Journal
Accession number :
22569252
Full Text :
https://doi.org/10.1016/j.febslet.2011.12.040