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Role of autophagy in monokine induced by interferon γ (Mig) production during adenovirus-hepatitis B virus infection.
- Source :
-
Hepato-gastroenterology [Hepatogastroenterology] 2012 Jun; Vol. 59 (116), pp. 1245-50. - Publication Year :
- 2012
-
Abstract
- Background/aims: The aim of this study was to investigate the effect of HBV-induced Mig and role of autophagy in the process.<br />Methodology: Adxsi-1.3 x HBV plasmid was constructed and identified. The three cell lines (L02, HepG2, SMMC-7721) were infected with adenovirus-HBV. HBsAg and HBeAg were assessed by electrochemiluminescence immunoassay. HBV DNA, HBx, Beclin 1 and Mig expression were detected by quantitative real-Time PCR, western blotting and ELISA. The level of autophagy was evaluated by transmission electron microscope.<br />Results: Human fetal liver cells and hepatocellular carcinoma cells were successfully transfected with overlength HBV genome using an adenovirus vector (Ad-HBV). Ad-HBV induced Mig production and cell autophagy through up-regulation of Beclin 1 expression. We further demonstrated that the increased autophagy extent was in association with HBV-induced Mig expression.<br />Conclusions: Autophagy may be a crucial intracellular mechanism of Mig induction in response to HBV infection. The results provide new insights into the pathogenesis of HBV.
- Subjects :
- Apoptosis Regulatory Proteins biosynthesis
Beclin-1
Cells, Cultured
Class III Phosphatidylinositol 3-Kinases physiology
DNA, Viral analysis
Genetic Vectors
Hep G2 Cells
Hepatitis B Surface Antigens analysis
Hepatitis B e Antigens analysis
Humans
Membrane Proteins biosynthesis
Trans-Activators analysis
Transfection
Viral Regulatory and Accessory Proteins
Adenoviridae genetics
Autophagy physiology
Chemokine CXCL9 biosynthesis
Hepatitis B virus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 0172-6390
- Volume :
- 59
- Issue :
- 116
- Database :
- MEDLINE
- Journal :
- Hepato-gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 22580676
- Full Text :
- https://doi.org/10.5754/hge12089