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[Effects of citreoviridin on the expression of MCP-1 and ILs induced by TNF-alpha in vein endothelial cells].
- Source :
-
Wei sheng yan jiu = Journal of hygiene research [Wei Sheng Yan Jiu] 2012 Mar; Vol. 41 (2), pp. 195-8, 203. - Publication Year :
- 2012
-
Abstract
- Objective: To investigate the effects of citreoviridin (CIT) on the expression of MCP-1, IL-1beta, IL-6 and IL-8 induced by TNF-alpha in human umbilical vein endothelial cells (HUVECs).<br />Methods: HUVECs isolated from the umbilical cord of neonates within 1 hour after birth (informed with consent form) were cultured in DMEM/ F12 media. After 80% of HUVECs were confluent, the cells were divided into four groups and treated with CIT (2 micromol/L) and/or TNF-alpha (10 microg/L). The levels of MCP-1, IL-1beta, IL-6 and IL-8 in the supernatant of cell culture media were measured by ELISA, the activation of NF-kappaB in HUVECs was detected by immunofluorescence staining, and the expression of MCP-1 mRNA was determinated by RT-PCR assay.<br />Result: The levels of MCP-1, IL-1beta, IL-6 and IL-8 in the supernatant and the expression of NF-kappaB P65 and MCP-1 mRNA of HUVECs were higher in TNF-alpha group and TNF-alpha + CIT group than those in the control group (P < 0.05), and those of TNF-alpha + CIT group was higher than TNF-alpha group (P < 0.05).<br />Conclusion: The expression of NF-kappaB, MCP-1, IL-1beta, IL-6 and IL-8 in HUVECs up-regulated by TNF-alpha was promoted by CIT.
- Subjects :
- Cells, Cultured
Chemokine CCL2 genetics
Human Umbilical Vein Endothelial Cells cytology
Human Umbilical Vein Endothelial Cells drug effects
Humans
Interleukin-1beta genetics
Interleukin-1beta metabolism
Interleukin-6 genetics
Interleukin-6 metabolism
Interleukin-8 genetics
Interleukin-8 metabolism
Interleukins genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Transcription Factor RelA genetics
Transcription Factor RelA metabolism
Aurovertins pharmacology
Chemokine CCL2 metabolism
Human Umbilical Vein Endothelial Cells metabolism
Interleukins metabolism
Tumor Necrosis Factor-alpha pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-8020
- Volume :
- 41
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Wei sheng yan jiu = Journal of hygiene research
- Publication Type :
- Academic Journal
- Accession number :
- 22611924