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Response of urinary biomarkers of systemic oxidation to oral iron supplementation in healthy men.
- Source :
-
Food and nutrition bulletin [Food Nutr Bull] 2012 Mar; Vol. 33 (1), pp. 53-62. - Publication Year :
- 2012
-
Abstract
- Background: Urinary biomarkers are used in assessment of severe, clinical oxidative stress. Little is known, however, about their diagnostic value within the normative range.<br />Objective: To evaluate the response of urinary thiobarbituric acid reactive substances (TBARS) and 8-hydroxy-2-deoxyguanosine (8-OHdG) as indicators of systemic oxidation in response to short-term oral iron and antioxidant supplementation.<br />Methods: Five healthy adult men participated in the pilot study phase and 12 in the definitive intervention trial. For 7 days each, separated by 12-day washouts, the subjects received different treatment regimens, consisting of 120 mg of iron, 120 mg of iron in refined palm oil, and 120 mg of iron in palm oil combined with one of the two doses of Carotino Tocotrienol Carotene Mixed Concentrate (CTCMC). Creatinine-normalized urinary TBARS and 8-OHdG concentrations were quantified in samples taken from subjects with and without active supplementation. Temporal and correlative associations between TBARS and 8-OHdG were explored.<br />Results: Daily intake of supplemental iron failed to produce any increment in urinary excretion of TBARS or 8-OHdG. However, a significant within-individual correlation between the urinary biomarkers was observed (Spearman r = 0.697, p < .0001, n = 466). Both doses of CTCMC significantly lowered urinary excretion of both oxidation indicators.<br />Conclusions: Despite the lack of effect of oral iron on the biomarkers of systemic oxidation, they show a strong and significant mutual association within the nonpathological range of oxidative stress in healthy male adults.
- Subjects :
- 8-Hydroxy-2'-Deoxyguanosine
Adolescent
Adult
Biomarkers urine
Carotenoids therapeutic use
Cross-Over Studies
Deoxyguanosine urine
Guatemala
Humans
Longitudinal Studies
Male
Oxidation-Reduction
Pilot Projects
Tocotrienols therapeutic use
Young Adult
Antioxidants therapeutic use
Deoxyguanosine analogs & derivatives
Dietary Supplements adverse effects
Ferrous Compounds adverse effects
Malondialdehyde urine
Oxidative Stress
Thiobarbituric Acid Reactive Substances analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0379-5721
- Volume :
- 33
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Food and nutrition bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 22624298
- Full Text :
- https://doi.org/10.1177/156482651203300105