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Lethality and cooperation of Pseudomonas aeruginosa quorum-sensing mutants in Drosophila melanogaster infection models.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2012 Aug; Vol. 158 (Pt 8), pp. 2125-2132. Date of Electronic Publication: 2012 May 24. - Publication Year :
- 2012
-
Abstract
- The virulence profiles of Pseudomonas aeruginosa quorum-sensing (QS) mutants were assessed in Drosophila melanogaster feeding and nicking infection models. Functional RhlIR and LasIR QS systems were required for killing in the fly feeding infection model but were not essential in the fly nicking infection model. Mixed infections between PAO1 and strains harbouring mutations in lasR, rhlI and lasI rhlI resulted in increased lethality in the fly feeding model compared with either isolate alone. These results suggested that the parental strain could cooperate with QS mutants in the Drosophila feeding infection model. Finally, the mixed infection between PAO1 and an rhlR mutant resulted in spiteful behaviour and reduced pathogenicity of the mixed culture.
- Subjects :
- Animals
Bacterial Proteins genetics
Bacterial Proteins metabolism
Disease Models, Animal
Gene Expression Regulation, Bacterial
Humans
Mutation
Pseudomonas aeruginosa genetics
Virulence
Drosophila melanogaster microbiology
Pseudomonas Infections microbiology
Pseudomonas Infections mortality
Pseudomonas aeruginosa pathogenicity
Pseudomonas aeruginosa physiology
Quorum Sensing
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2080
- Volume :
- 158
- Issue :
- Pt 8
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 22628480
- Full Text :
- https://doi.org/10.1099/mic.0.054999-0