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CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2012 Oct; Vol. 72 (2), pp. 185-98. Date of Electronic Publication: 2012 Aug 23. - Publication Year :
- 2012
-
Abstract
- The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.<br /> (© 2012 Institut National de la Recherche Agronomic. The Plant Journal © 2012 Blackwell Publishing Ltd.)
- Subjects :
- Animals
Arabidopsis cytology
Arabidopsis metabolism
Arabidopsis parasitology
Arabidopsis Proteins genetics
Cell Cycle
Cell Cycle Proteins genetics
Cell Cycle Proteins metabolism
Down-Regulation
Endoreduplication
Female
Gene Expression Regulation, Plant
Gene Knockdown Techniques
Gene Knockout Techniques
Giant Cells metabolism
Giant Cells parasitology
Plant Roots cytology
Plant Roots genetics
Plant Roots parasitology
Plants, Genetically Modified
Ploidies
Real-Time Polymerase Chain Reaction
Seedlings cytology
Seedlings genetics
Seedlings metabolism
Seedlings parasitology
Transcription Factors genetics
Transcription Factors metabolism
Tylenchoidea cytology
Arabidopsis genetics
Arabidopsis Proteins metabolism
Plant Diseases parasitology
Tylenchoidea physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 72
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 22640471
- Full Text :
- https://doi.org/10.1111/j.1365-313X.2012.05054.x