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Increased expression of fractalkine and its receptor CX3CR1 in canine inflammatory bowel disease and their possible role in recruitment of intraepithelial lymphocytes.
- Source :
-
Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2012 Aug 15; Vol. 148 (3-4), pp. 226-35. Date of Electronic Publication: 2012 May 09. - Publication Year :
- 2012
-
Abstract
- The interaction between fractalkine/CX(3)CL1 and its receptor CX(3)CR1 has been reported to play an important role in various human inflammatory diseases, including inflammatory bowel disease (IBD) mediated by lymphocyte chemoattraction. The objective of this study was to investigate the role of fractalkine and CX(3)CR1 in lymphocyte migration in canine IBD. IBD was diagnosed in 34 dogs, and 19 healthy beagles were used as normal controls. We quantified intestinal mRNA and protein expression of fractalkine and CX(3)CR1 by real-time RT-PCR and ELISA, respectively, and examined the localization of fractalkine in canine intestine by immunohistochemistry. The expression of CX(3)CR1 and surface antigens on peripheral blood mononuclear cells (PBMCs) and intraepithelial lymphocytes (IELs) was analyzed by flow cytometry. Intestinal fractalkine and CX(3)CR1 mRNA was significantly up-regulated in IBD dogs compared with the healthy control dogs. In addition, fractalkine expression on intestinal epithelial cells was significantly increased in the intestinal mucosa of IBD dogs compared with the healthy dogs. CX(3)CR1(+) PBMCs were significantly elevated in IBD dogs and positively correlated with the histopathological severity of IELs infiltration. These CX(3)CR1(+) PBMCs predominantly expressed markers for cytotoxic T cells. Almost all IELs expressed CD3, and the majority of cells expressed CD8 rather than CD4, which was analogous to the CX(3)CR1(+) PBMCs. These results suggest that the fractalkine-CX(3)CR1 interaction may contribute to the pathogenesis of canine IBD through migration of IELs.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Movement immunology
Dogs
Duodenum immunology
Duodenum metabolism
Enzyme-Linked Immunosorbent Assay veterinary
Female
Flow Cytometry veterinary
Inflammatory Bowel Diseases immunology
Intestinal Mucosa metabolism
Male
Real-Time Polymerase Chain Reaction veterinary
Chemokine CX3CL1 biosynthesis
Dog Diseases immunology
Inflammatory Bowel Diseases veterinary
Intestinal Mucosa immunology
Lymphocytes immunology
Receptors, Chemokine biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2534
- Volume :
- 148
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary immunology and immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 22648046
- Full Text :
- https://doi.org/10.1016/j.vetimm.2012.04.021