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Development of a diagnostic method applicable to various serotypes of hantavirus infection in rodents.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2012 Sep; Vol. 74 (9), pp. 1237-42. Date of Electronic Publication: 2012 May 21. - Publication Year :
- 2012
-
Abstract
- Antigenic diversity among different hantaviruses requires a variety of reagents for diagnosis of hantavirus infection. To develop a diagnostic method applicable to various hantavirus infections with a single set of reagents, we developed an enzyme-linked immunosorbent assay (ELISA) using recombinant nucleocapsid proteins of three hantaviruses, Amur, Hokkaido, and Sin Nombre viruses. This novel cocktail antigen-based ELISA enabled detection of antibodies against Hantaan, Seoul, Amur, Puumala, and Sin Nombre viruses in immunized laboratory animals. In wild rodent species, including Apodemus, Rattus, and Myodes, our ELISA detected antibodies against hantaviruses with high sensitivity and specificity. These data suggest that our novel diagnostic ELISA is a useful tool for screening hantavirus infections and could be effectively utilized for serological surveillance and quarantine purposes.
- Subjects :
- Animals
Antibodies, Viral isolation & purification
Enzyme-Linked Immunosorbent Assay methods
Hantavirus Infections diagnosis
Hantavirus Infections immunology
Nucleocapsid Proteins genetics
Recombinant Proteins genetics
Rodent Diseases immunology
Sensitivity and Specificity
Serologic Tests veterinary
Species Specificity
Arvicolinae
Enzyme-Linked Immunosorbent Assay veterinary
Orthohantavirus genetics
Hantavirus Infections veterinary
Murinae
Rats
Rodent Diseases diagnosis
Rodent Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 74
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 22673703
- Full Text :
- https://doi.org/10.1292/jvms.12-0006