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One-step derivation of cardiomyocytes and mesenchymal stem cells from human pluripotent stem cells.
- Source :
-
Stem cell research [Stem Cell Res] 2012 Sep; Vol. 9 (2), pp. 87-100. Date of Electronic Publication: 2012 Apr 24. - Publication Year :
- 2012
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Abstract
- Cardiomyocytes (CMs) and mesenchymal stem cells (MSCs) are important cell types for cardiac repair post myocardial infarction. Here we proved that both CMs and MSCs can be simultaneously generated from human induced pluripotent stem cells (hiPSCs) via a pro-mesoderm differentiation strategy. Two hiPSC lines, hiPSC (1) and hiPSC (2) were generated from human dermal fibroblasts using OCT-4, SOX-2, KLF-4, c-Myc via retroviral-based reprogramming. H9 human embryonic stem cells (hESCs) served as control. CMs and MSCs were co-generated from hiPSCs and hESCs via embryoid body-dependent cardiac differentiation protocol involving a serum-free and insulin-depleted medium containing a p38 MAPK inhibitor, SB 203580. Comparing to bone marrow and umbilical cord blood-derived MSCs, hiPSC-derived MSCs (iMSCs) expressed common MSC markers and were capable of adipogenesis, osteogenesis and chondrogenesis. Moreover, iMSCs continuously proliferated for more than 32 population doublings without cellular senescence and showed superior pro-angiogenic and wound healing properties. In summary, we generated a large number of homogenous MSCs in conjunction with CMs in a low-cost and efficient one step manner. Functionally competent CMs and MSCs co-generated from hiPSCs may be useful for autologous cardiac repair.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Biomarkers metabolism
Cell Differentiation
Cell Line
Cell Lineage
Cell Membrane metabolism
Cell Proliferation
Cell Transformation, Neoplastic pathology
Chromosome Aberrations
Female
Flow Cytometry
Humans
Karyotyping
Kinetics
Mesenchymal Stem Cells metabolism
Mice
Mice, SCID
Multipotent Stem Cells cytology
Myocytes, Cardiac physiology
Neovascularization, Physiologic
Telomerase metabolism
Wound Healing
Cell Culture Techniques methods
Induced Pluripotent Stem Cells cytology
Mesenchymal Stem Cells cytology
Myocytes, Cardiac cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1876-7753
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Stem cell research
- Publication Type :
- Academic Journal
- Accession number :
- 22683798
- Full Text :
- https://doi.org/10.1016/j.scr.2012.04.003