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A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (6), pp. e38718. Date of Electronic Publication: 2012 Jun 08. - Publication Year :
- 2012
-
Abstract
- A key enzyme in the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the primary target of the broad-spectrum herbicide glyphosate. Identification of new aroA genes coding for EPSPS with a high level of glyphosate tolerance is essential for the development of glyphosate-tolerant crops. In the present study, the glyphosate tolerance of five bacterial aroA genes was evaluated in the E. coli aroA-defective strain ER2799 and in transgenic tobacco plants. All five aroA genes could complement the aroA-defective strain ER2799, and AM79 aroA showed the highest glyphosate tolerance. Although glyphosate treatment inhibited the growth of both WT and transgenic tobacco plants, transgenic plants expressing AM79 aroA tolerated higher concentration of glyphosate and had a higher fresh weight and survival rate than plants expressing other aroA genes. When treated with high concentration of glyphosate, lower shikimate content was detected in the leaves of transgenic plants expressing AM79 aroA than transgenic plants expressing other aroA genes. These results suggest that AM79 aroA could be a good candidate for the development of transgenic glyphosate-tolerant crops.
- Subjects :
- 3-Phosphoshikimate 1-Carboxyvinyltransferase genetics
Animals
Drug Resistance, Bacterial genetics
Escherichia coli genetics
Escherichia coli Proteins genetics
Glycine pharmacology
Plants, Genetically Modified genetics
Nicotiana genetics
Glyphosate
3-Phosphoshikimate 1-Carboxyvinyltransferase biosynthesis
Drug Resistance, Bacterial drug effects
Escherichia coli enzymology
Escherichia coli Proteins biosynthesis
Glycine analogs & derivatives
Herbicides pharmacology
Plants, Genetically Modified enzymology
Nicotiana enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22715408
- Full Text :
- https://doi.org/10.1371/journal.pone.0038718