Back to Search
Start Over
Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells.
- Source :
-
Protein & cell [Protein Cell] 2012 Aug; Vol. 3 (8), pp. 618-26. Date of Electronic Publication: 2012 Jun 22. - Publication Year :
- 2012
-
Abstract
- Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca(2+) entry and suppressed by the inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice, which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability.
- Subjects :
- Animals
Calcium metabolism
Diabetes Mellitus pathology
Disease Models, Animal
Dynamins metabolism
Electric Capacitance
Insulin-Secreting Cells pathology
Male
Mice
Mice, Inbred C57BL
Patch-Clamp Techniques
Photolysis
Primary Cell Culture
Cytoplasmic Granules metabolism
Diabetes Mellitus metabolism
Endocytosis physiology
Insulin metabolism
Insulin-Secreting Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1674-8018
- Volume :
- 3
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Protein & cell
- Publication Type :
- Academic Journal
- Accession number :
- 22729398
- Full Text :
- https://doi.org/10.1007/s13238-012-2938-0