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In vitro trans-translation assays.

Authors :
Kurita D
Muto A
Himeno H
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 905, pp. 311-25.
Publication Year :
2012

Abstract

Trans-translation is a bacterial quality control system in protein synthesis facilitated by transfer-messenger RNA (tmRNA). Here, we describe the in vitro system using purified factors to evaluate the two steps of trans-translation: peptidyl-transfer from peptidyl-tRNA to alanyl-tmRNA and decoding of the resume codon on tmRNA.

Subjects

Subjects :
Amino Acyl-tRNA Synthetases metabolism
Codon genetics
Codon metabolism
Escherichia coli cytology
Peptide Elongation Factors metabolism
RNA, Bacterial metabolism
RNA, Transfer, Amino Acyl metabolism
Ribosomes genetics
Ribosomes metabolism
Protein Biosynthesis

Details

Language :
English
ISSN :
1940-6029
Volume :
905
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
22736013
Full Text :
https://doi.org/10.1007/978-1-61779-949-5_20
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