A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra.

Authors :: Gardiennet C
Schütz AK
Hunkeler A
Kunert B
Terradot L
Böckmann A
Meier BH
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2012 Jul 27; Vol. 51 (31), pp. 7855-8. Date of Electronic Publication: 2012 Jun 27.
Publication Year :: 2012
Abstract: Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.<br /> (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: DnaB Helicases metabolism
DnaB Helicases chemistry
Nuclear Magnetic Resonance, Biomolecular

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