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Gef gene therapy enhances the therapeutic efficacy of cytotoxics in colon cancer cells.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2012 Oct; Vol. 66 (7), pp. 563-7. Date of Electronic Publication: 2012 Jun 19. - Publication Year :
- 2012
-
Abstract
- The potential use of gene therapy to improve the response of patients with advanced cancer is being intensively analyzed. We evaluated the cytotoxic impact of the gef gene, a suicide gene, which has a demonstrated antiproliferative activity in tumor cells, in colon carcinoma cells in order to improve the antitumour effect of chemotherapeutic drugs used as first line treatment in the management of advanced colon cancer. We found that the gef gene induced a marked decrease in cell viability (50% in 24h) in T-84 cells through cell death by apoptosis. Interestingly, when gef gene expression was combined with drugs of choice in the clinical treatment of colon cancer (5-fluorouracil, oxaliplatin and irinotecan), a strong synergistic effect was observed with approximately a 15-20% enhancement of the antiproliferative effect. Our data demonstrate, for the first time, that gef gene expression induces significant growth arrest in colon cancer cells and that it is able to enhance the effect of some cytotoxic drugs compared with a single therapeutic approach. These results indicate the potential therapeutic value of the gef gene in colon cancer combination therapy.<br /> (Copyright © 2012 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Apoptosis drug effects
Apoptosis genetics
Camptothecin analogs & derivatives
Camptothecin pharmacology
Cell Survival drug effects
Cell Survival genetics
Colonic Neoplasms pathology
Combined Modality Therapy
Fluorouracil pharmacology
Humans
Irinotecan
Organoplatinum Compounds pharmacology
Oxaliplatin
Antineoplastic Agents pharmacology
Bacterial Toxins genetics
Colonic Neoplasms therapy
Escherichia coli Proteins genetics
Genetic Therapy methods
Membrane Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 66
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 22770988
- Full Text :
- https://doi.org/10.1016/j.biopha.2012.05.004