Back to Search
Start Over
Inhibition of long myosin light-chain kinase activation alleviates intestinal damage after binge ethanol exposure and burn injury.
- Source :
-
American journal of physiology. Gastrointestinal and liver physiology [Am J Physiol Gastrointest Liver Physiol] 2012 Sep 15; Vol. 303 (6), pp. G705-12. Date of Electronic Publication: 2012 Jul 12. - Publication Year :
- 2012
-
Abstract
- Laboratory evidence suggests that intestinal permeability is elevated following either binge ethanol exposure or burn injury alone, and this barrier dysfunction is further perturbed when these insults are combined. We and others have previously reported a rise in both systemic and local proinflammatory cytokine production in mice after the combined insult. Knowing that long myosin light-chain kinase (MLCK) is important for epithelial barrier maintenance and can be activated by proinflammatory cytokines, we examined whether inhibition of MLCK alleviated detrimental intestinal responses seen after ethanol exposure and burn injury. To accomplish this, mice were given vehicle or a single binge ethanol exposure followed by a sham or dorsal scald burn injury. Following injury, one group of mice received membrane permeant inhibitor of MLCK (PIK). At 6 and 24 h postinjury, bacterial translocation and intestinal levels of proinflammatory cytokines were measured, and changes in tight junction protein localization and total intestinal morphology were analyzed. Elevated morphological damage, ileal IL-1β and IL-6 levels, and bacterial translocation were seen in mice exposed to ethanol and burn injury relative to either insult alone. This increase was not seen in mice receiving PIK after injury. Ethanol-exposed and burn-injured mice had reduced zonula occludens protein-1 and occludin localization to the tight junction relative to sham-injured mice. However, the observed changes in junctional complexes were not seen in our PIK-treated mice following the combined insult. These data suggest that MLCK activity may promote morphological and inflammatory responses in the ileum following ethanol exposure and burn injury.
- Subjects :
- Animals
Gene Expression Regulation, Enzymologic
Inflammation prevention & control
Interleukin-6 genetics
Interleukin-6 metabolism
Male
Mice
Mice, Inbred C57BL
Myosin-Light-Chain Kinase metabolism
Zonula Occludens-1 Protein genetics
Zonula Occludens-1 Protein metabolism
Burns pathology
Ethanol administration & dosage
Ethanol toxicity
Intestinal Diseases chemically induced
Intestinal Diseases drug therapy
Myosin-Light-Chain Kinase antagonists & inhibitors
Peptides therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1547
- Volume :
- 303
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Publication Type :
- Academic Journal
- Accession number :
- 22790598
- Full Text :
- https://doi.org/10.1152/ajpgi.00157.2012