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Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2012; Vol. 7, pp. 3099-109. Date of Electronic Publication: 2012 Jun 29. - Publication Year :
- 2012
-
Abstract
- Background: Recent studies have shown that the biological actions and toxicity of the water-soluble compound, polyhydroxyfullerene (fullerenol), are related to the concentrations present at a particular site of action. This study investigated the effects of different concentrations of fullerenol on cultured rat hippocampal neurons.<br />Methods and Results: Fullerenol at low concentrations significantly enhanced hippocampal neuron viability as tested by MTT assay and Hoechst 33342/propidium iodide double stain detection. At high concentrations, fullerenol induced apoptosis confirmed by Comet assay and assessment of caspase proteins.<br />Conclusion: These findings suggest that fullerenol promotes cell death and protects against cell damage, depending on the concentration present. The concentration-dependent effects of fullerenol were mainly due to its influence on the reduction-oxidation pathway.
- Subjects :
- Aldehydes metabolism
Animals
Apoptosis drug effects
Caspases metabolism
Cell Survival drug effects
Comet Assay
Dose-Response Relationship, Drug
Fullerenes toxicity
Glutathione metabolism
Hippocampus cytology
Lead toxicity
Neurons cytology
Neurons metabolism
Neuroprotective Agents pharmacology
Neuroprotective Agents toxicity
Neurotoxins pharmacology
Neurotoxins toxicity
Oxidative Stress
Rats
Rats, Wistar
Superoxide Dismutase metabolism
Fullerenes pharmacology
Hippocampus drug effects
Neurons drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 22802681
- Full Text :
- https://doi.org/10.2147/IJN.S30934