An integrated chip for the high-throughput synthesis of transcription activator-like effectors.

Authors :: Wang Z
Li J
Huang H
Wang G
Jiang M
Yin S
Sun C
Zhang H
Zhuang F
Xi JJ
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2012 Aug 20; Vol. 51 (34), pp. 8505-8. Date of Electronic Publication: 2012 Jul 23.
Publication Year :: 2012
Abstract: A TALE of two assays: Transcription activator-like effectors (TALEs) are programmable proteins that can specifically recognize a DNA sequence. Previous strategies for the synthesis of TALEs were complicated and time-consuming. The solid-phase synthesis strategy demonstrated here allows quick and simple purification of the ligation product.<br /> (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: HeLa Cells
Humans
Protein Engineering
Trans-Activators genetics
Trans-Activators metabolism
Transcription Factors genetics
Transcription Factors metabolism
High-Throughput Screening Assays methods
Trans-Activators chemical synthesis
Transcription Factors chemical synthesis

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