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A NAC transcription factor and SNI1 cooperatively suppress basal pathogen resistance in Arabidopsis thaliana.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2012 Oct; Vol. 40 (18), pp. 9182-92. Date of Electronic Publication: 2012 Jul 22. - Publication Year :
- 2012
-
Abstract
- Transcriptional repression of pathogen defense-related genes is essential for plant growth and development. Several proteins are known to be involved in the transcriptional regulation of plant defense responses. However, mechanisms by which expression of defense-related genes are regulated by repressor proteins are poorly characterized. Here, we describe the in planta function of CBNAC, a calmodulin-regulated NAC transcriptional repressor in Arabidopsis. A T-DNA insertional mutant (cbnac1) displayed enhanced resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae DC3000 (PstDC3000), whereas resistance was reduced in transgenic CBNAC overexpression lines. The observed changes in disease resistance were correlated with alterations in pathogenesis-related protein 1 (PR1) gene expression. CBNAC bound directly to the PR1 promoter. SNI1 (suppressor of nonexpressor of PR genes1, inducible 1) was identified as a CBNAC-binding protein. Basal resistance to PstDC3000 and derepression of PR1 expression was greater in the cbnac1 sni1 double mutant than in either cbnac1 or sni1 mutants. SNI1 enhanced binding of CBNAC to its cognate PR1 promoter element. CBNAC and SNI1 are hypothesized to work as repressor proteins in the cooperative suppression of plant basal defense.
- Subjects :
- Arabidopsis metabolism
Arabidopsis Proteins biosynthesis
Arabidopsis Proteins genetics
DNA metabolism
Gene Expression Regulation, Plant
Plant Diseases microbiology
Promoter Regions, Genetic
Pseudomonas syringae
RNA, Messenger biosynthesis
Repressor Proteins biosynthesis
Repressor Proteins genetics
Salicylic Acid
Arabidopsis genetics
Arabidopsis microbiology
Arabidopsis Proteins metabolism
Disease Resistance genetics
Nuclear Proteins metabolism
Plant Diseases genetics
Repressor Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 40
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 22826500
- Full Text :
- https://doi.org/10.1093/nar/gks683