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Optimization and characterization of a polysaccharide produced by Pseudomonas fluorescens WR-1 and its antioxidant activity.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2012 Oct 01; Vol. 90 (2), pp. 921-9. Date of Electronic Publication: 2012 Jun 18. - Publication Year :
- 2012
-
Abstract
- The extracellular polysaccharide produced by a newly isolated strain Pseudomonas fluorescens WR-1 was purified and characterized and its production was optimized using response surface methodology. The results showed that the strain WR-1 produced one kind of EPS that was composed of arabinose, glucose and uronic acid. The molecular weight of the EPS was determined to be 6.78 × 10(6)Da. The preferable culture conditions for EPS production were pH 7.0, temperature 28°C for 72 h with peptone and maltose as best N and C sources, respectively. The model predicted that the maximum EPS production (39.6 gL(-1)) was appeared with maltose 48.65 gL(-1), Mn(2+) 1118 μM and Zn(2+) 901 μM. The EPS also showed good H(2)O(2) scavenging activity while moderate free radical scavenging activity and reductive ability were determined. The EPS from WR-1 may be a new source of natural antioxidants with potential value for health, food and industry.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Antioxidants chemistry
Antioxidants pharmacology
Calibration
Chemical Fractionation
Drug Industry methods
Drug Industry standards
Free Radical Scavengers chemistry
Free Radical Scavengers isolation & purification
Free Radical Scavengers pharmacology
Free Radicals metabolism
Hydrogen Peroxide metabolism
Molecular Weight
Oxidation-Reduction drug effects
Polysaccharides, Bacterial chemistry
Antioxidants isolation & purification
Polysaccharides, Bacterial isolation & purification
Polysaccharides, Bacterial pharmacology
Pseudomonas fluorescens chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 90
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 22840021
- Full Text :
- https://doi.org/10.1016/j.carbpol.2012.06.021