Back to Search
Start Over
A novel HIV vaccine adjuvanted by IC31 induces robust and persistent humoral and cellular immunity.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (7), pp. e42163. Date of Electronic Publication: 2012 Jul 25. - Publication Year :
- 2012
-
Abstract
- The HIV vaccine strategy that, to date, generated immune protection consisted of a prime-boost regimen using a canarypox vector and an HIV envelope protein with alum, as shown in the RV144 trial. Since the efficacy was weak, and previous HIV vaccine trials designed to generate antibody responses failed, we hypothesized that generation of T cell responses would result in improved protection. Thus, we tested the immunogenicity of a similar envelope-based vaccine using a mouse model, with two modifications: a clade C CN54gp140 HIV envelope protein was adjuvanted by the TLR9 agonist IC31®, and the viral vector was the vaccinia strain NYVAC-CN54 expressing HIV envelope gp120. The use of IC31® facilitated immunoglobulin isotype switching, leading to the production of Env-specific IgG2a, as compared to protein with alum alone. Boosting with NYVAC-CN54 resulted in the generation of more robust Th1 T cell responses. Moreover, gp140 prime with IC31® and alum followed by NYVAC-CN54 boost resulted in the formation and persistence of central and effector memory populations in the spleen and an effector memory population in the gut. Our data suggest that this regimen is promising and could improve the protection rate by eliciting strong and long-lasting humoral and cellular immune responses.
- Subjects :
- AIDS Vaccines genetics
Alum Compounds pharmacology
Animals
CD4-Positive T-Lymphocytes drug effects
CD4-Positive T-Lymphocytes immunology
Drug Combinations
Female
Genetic Vectors genetics
HIV Envelope Protein gp120 genetics
HIV Envelope Protein gp120 immunology
Immunization, Secondary
Immunologic Memory drug effects
Immunologic Memory immunology
Mesentery immunology
Mice
Vaccinia virus genetics
env Gene Products, Human Immunodeficiency Virus genetics
env Gene Products, Human Immunodeficiency Virus immunology
AIDS Vaccines immunology
Adjuvants, Immunologic pharmacology
Immunity, Cellular drug effects
Immunity, Cellular immunology
Immunity, Humoral drug effects
Immunity, Humoral immunology
Oligodeoxyribonucleotides immunology
Oligopeptides immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22848738
- Full Text :
- https://doi.org/10.1371/journal.pone.0042163