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Hypoglycaemic effect of Melothria heterophylla in streptozotocin-induced diabetic rats.
- Source :
-
Pharmaceutical biology [Pharm Biol] 2012 Sep; Vol. 50 (9), pp. 1151-6. Date of Electronic Publication: 2012 Jul 31. - Publication Year :
- 2012
-
Abstract
- Context: In the Indian traditional system of medicine, Melothria heterophylla (Lour.) Cogn., (Cucurbitaceae) is prescribed for the treatment of diabetes mellitus.<br />Objective: In the present study, the antidiabetic effect of ethanol extract of Melothria heterophylla (EEMH), and its active isolated constituents were investigated in streptozotocin (STZ)-induced diabetic Swiss albino rats.<br />Method: Successive Soxhlet extraction of the dried total aerial parts with petroleum ether for defatting and then with ethanol (95%) to obtain ethanol extract, which was concentrated under reduced pressure. Hyperglycemia was induced in rats by STZ (50 mg/kg, body weight). Twenty-four hours after STZ induction, respective groups of diabetic rats received EEMH (200 and 400 mg/kg, body weight), gallic acid (GA) (2 and 4 mg/kg, body weight), and rutin (RU) (2 and 4 mg/kg, body weight), respectively, orally daily for 15 days. Glibenclamide (0.5 mg/kg, orally) served as reference. Blood glucose levels and change in body weight were measured on every 5(th) day during 15 days of treatment. Biochemical parameters, viz., serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and serum insulin, were measured.<br />Results: EEMH and its active constituents significantly (p < 0.01) normalized blood glucose levels and serum biochemical parameters as compared to those of STZ controls. Both GA (4 mg/kg) and RU (4 mg/kg) exhibited maximum glucose lowering effect (69.1 and 66.7%, respectively) in diabetic rats compared to the other dose (2 mg/kg) at the end of the study. EEMH, gallic acid and RU also showed significant increase in serum insulin, and body weight of STZ-induced diabetic rats.<br />Conclusion: Therefore, ethanol extract of Melothria heterophylla, GA and RU demonstrated remarkable antidiabetic activity in STZ-induced diabetic rats.
- Subjects :
- Animals
Diabetes Mellitus, Experimental blood
Diabetes Mellitus, Experimental complications
Diabetes Mellitus, Experimental physiopathology
Ethnopharmacology
Gallic Acid administration & dosage
Gallic Acid adverse effects
Gallic Acid isolation & purification
Gallic Acid therapeutic use
Hepatic Insufficiency complications
Hepatic Insufficiency prevention & control
Hypoglycemic Agents administration & dosage
Hypoglycemic Agents adverse effects
Hypoglycemic Agents isolation & purification
India
Insulin blood
Insulin metabolism
Insulin Secretion
Liver drug effects
Liver physiopathology
Male
Plant Extracts administration & dosage
Plant Extracts adverse effects
Plant Extracts isolation & purification
Rats
Rutin administration & dosage
Rutin adverse effects
Rutin isolation & purification
Rutin therapeutic use
Streptozocin
Toxicity Tests, Acute
Weight Loss drug effects
Cucurbitaceae chemistry
Diabetes Mellitus, Experimental drug therapy
Hyperglycemia prevention & control
Hypoglycemic Agents therapeutic use
Phytotherapy
Plant Components, Aerial chemistry
Plant Extracts therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1744-5116
- Volume :
- 50
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Pharmaceutical biology
- Publication Type :
- Academic Journal
- Accession number :
- 22849439
- Full Text :
- https://doi.org/10.3109/13880209.2012.661742