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Lotus japonicus ARPC1 is required for rhizobial infection.
- Source :
-
Plant physiology [Plant Physiol] 2012 Oct; Vol. 160 (2), pp. 917-28. Date of Electronic Publication: 2012 Aug 03. - Publication Year :
- 2012
-
Abstract
- Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.
- Subjects :
- Actin Cytoskeleton genetics
Actin Cytoskeleton metabolism
Actin-Related Protein 2-3 Complex genetics
Actin-Related Protein 2-3 Complex metabolism
Agrobacterium tumefaciens genetics
Agrobacterium tumefaciens metabolism
Cloning, Molecular
Gene Expression Regulation, Plant
Genes, Plant
Genetic Complementation Test
Genetic Loci
Lotus genetics
Lotus growth & development
Lotus microbiology
Mutation
Mycorrhizae growth & development
Phenotype
Plant Epidermis metabolism
Plant Epidermis microbiology
Plant Proteins genetics
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Plants, Genetically Modified microbiology
RNA, Messenger genetics
RNA, Messenger metabolism
Root Nodules, Plant microbiology
Seeds genetics
Seeds metabolism
Symbiosis
Lotus metabolism
Mesorhizobium growth & development
Plant Proteins metabolism
Root Nodules, Plant growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 160
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 22864583
- Full Text :
- https://doi.org/10.1104/pp.112.202572