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Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose.

Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose.

Authors :
Sato H
Saburi W
Ojima T
Taguchi H
Mori H
Matsui H
Source :
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2012; Vol. 76 (8), pp. 1584-7. Date of Electronic Publication: 2012 Aug 07.
Publication Year :
2012

Abstract

Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) was supplied to the reactor, kept at 50 °C at a space velocity of 8 h(-1). The epilactose concentration of the resulting eluate was 30 g/L, and this was maintained for 13 d.

Details

Language :
English
ISSN :
1347-6947
Volume :
76
Issue :
8
Database :
MEDLINE
Journal :
Bioscience, biotechnology, and biochemistry
Publication Type :
Academic Journal
Accession number :
22878201
Full Text :
https://doi.org/10.1271/bbb.120284