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Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose.
Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2012; Vol. 76 (8), pp. 1584-7. Date of Electronic Publication: 2012 Aug 07. - Publication Year :
- 2012
-
Abstract
- Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) was supplied to the reactor, kept at 50 °C at a space velocity of 8 h(-1). The epilactose concentration of the resulting eluate was 30 g/L, and this was maintained for 13 d.
- Subjects :
- Bacterial Proteins genetics
Biocatalysis
Bioreactors
Cellobiose chemistry
Enzyme Stability
Escherichia coli genetics
Hot Temperature
Hydrogen-Ion Concentration
Immobilized Proteins genetics
Kinetics
Lactose chemistry
Racemases and Epimerases genetics
Recombinant Proteins chemistry
Recombinant Proteins genetics
Rhodothermus chemistry
Substrate Specificity
Bacterial Proteins chemistry
Disaccharides chemical synthesis
Immobilized Proteins chemistry
Racemases and Epimerases chemistry
Rhodothermus enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 76
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22878201
- Full Text :
- https://doi.org/10.1271/bbb.120284