[Construction of a red fluorescent shuttle vector controlled by recA operon promoter of Streptococcus mutans].

Objective: To construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans.
Methods: The promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred.
Results: pLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed.
Conclusions: The recombination plasmid pLRred can be used in the further research of the expression of cariogenic virulence factor gene by Streptococcus mutans in biofilm.