Back to Search
Start Over
Ginsenoside-Rb1 promotes adipogenesis through regulation of PPARγ and microRNA-27b.
- Source :
-
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme [Horm Metab Res] 2012 Oct; Vol. 44 (11), pp. 819-24. Date of Electronic Publication: 2012 Aug 14. - Publication Year :
- 2012
-
Abstract
- Ginsenoside-Rb1 (Rb1), one of the bioactive components in ginseng extract, is recently reported to be able to promote adipogenesis and peroxisome proliferator-activated receptor gamma (PPARγ) expression. Meanwhile, microRNA-27b (miR-27b) is also identified to regulate adipogenesis by targeting PPARγ2. In the present study, we attempted to link up the Rb1-promoted adipogenesis with PPARγ binding and miR-27b regulation. First, we demonstrated that GW9662, an antagonist of PPARγ, could block Rb1-induced 3T3-L1 differentiation with little toxicity towards cell proliferation. Then, expression levels for both of miR-27b and its primary transcript, pri-mir-27b, were found to decrease upon Rb1 treatment. Again, GW9662 could attenuate the inhibitory effect of Rb1 on both miR-27 and pri-mir-27b expression. Since Rb1 was demonstrated to have binding activity towards PPARγ, we thus speculate that Rb1 may act though PPARγ to downregulate mir-27b gene transcription and mature miR-27b activity, which in turn promotes PPARγ expression and adipogenesis. Enhancement on adipogenesis of adipose tissues is expected to prevent lipotoxicty in nonadipose tissues. Our data may give a better illustration to explain the antidiabetic effect of Rb1 and provide a hint on treatment of lipid related metabolic diseases in the future.<br /> (© Georg Thieme Verlag KG Stuttgart · New York.)
- Subjects :
- 3T3-L1 Cells
Adipocytes cytology
Adipocytes drug effects
Adipocytes metabolism
Animals
Gene Expression Regulation drug effects
Mice
MicroRNAs metabolism
PPAR gamma metabolism
Adipogenesis drug effects
Ginsenosides pharmacology
MicroRNAs genetics
PPAR gamma genetics
Plant Extracts pharmacology
Up-Regulation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1439-4286
- Volume :
- 44
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
- Publication Type :
- Academic Journal
- Accession number :
- 22893262
- Full Text :
- https://doi.org/10.1055/s-0032-1321909