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αA crystallin may protect against geographic atrophy-meta-analysis of cataract vs. cataract surgery for geographic atrophy and experimental studies.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (8), pp. e43173. Date of Electronic Publication: 2012 Aug 20. - Publication Year :
- 2012
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Abstract
- Background: Cataract and geographic atrophy (GA, also called advanced "dry" age-related macular degeneration) are the two major causes of visual impairment in the developed world. The association between cataract surgery and the development of GA was controversial in previous studies.<br />Methods/principal Findings: We performed a meta-analysis by pooling the current evidence in literature and found that cataract is associated with an increased risk of geographic atrophy with a summary odds ratio (OR) of 3.75 (95% CI: 95% CI: 1.84-7.62). However, cataract surgery is not associated with the risk of geographic atrophy (polled OR=3.23, 95% CI: 0.63-16.47). Further experiments were performed to analyze how the αA-crystallin, the major component of the lens, influences the development of GA in a mouse model. We found that theαA-crystallin mRNA and protein expression increased after oxidative stress induced by NaIO(3) in immunohistochemistry of retinal section and western blot of posterior eyecups. Both functional and histopathological evidence confirmed that GA is more severe in αA-crystallin knockout mice compared to wild-type mice.<br />Conclusions: Therefore, αA-crystallin may protect against geographic atrophy. This study provides a better understanding of the relationship between cataract, cataract surgery, and GA.
- Subjects :
- Animals
Blotting, Western
Cataract genetics
Cells, Cultured
Electroretinography
Geographic Atrophy genetics
Humans
Immunohistochemistry
Iodates pharmacology
Mice
Mice, Knockout
Oxidative Stress drug effects
Reactive Oxygen Species metabolism
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
alpha-Crystallin A Chain genetics
Cataract metabolism
Cataract Extraction
Geographic Atrophy metabolism
Geographic Atrophy surgery
alpha-Crystallin A Chain metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22916220
- Full Text :
- https://doi.org/10.1371/journal.pone.0043173