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Characterization of vitellogenin and its derived yolk proteins in cloudy catshark (Scyliorhinus torazame).
- Source :
-
Fish physiology and biochemistry [Fish Physiol Biochem] 2013 Apr; Vol. 39 (2), pp. 373-90. Date of Electronic Publication: 2012 Aug 24. - Publication Year :
- 2013
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Abstract
- Elasmobranchs (sharks and rays) exhibit unique reproductive characteristics and, in contrast to the situation in teleosts, very little is known about the identity, structure and physical characteristics of their egg yolk proteins. The aims of this study were to (1) detect and purify the vitellogenin (Vtg; egg yolk precursor) and yolk proteins (YPs) of the cloudy catshark (Scyliorhinus torazame), (2) examine the relationships between Vtg and YPs and (3) characterize and classify the deduced primary structure of the Vtg transcript (vtg). The apparent molecular weights of purified Vtg and putative Vtg-related YPs (lipovitellin: Lv, phosvitin: Pv) were determined by gel filtration and were ~560, >669 and ~58 kDa, respectively. Following SDS-PAGE, these purified products (i.e., Vtg, Lv and Pv) appeared as bands of ~210, ~110 and ~22 kDa, respectively. On Western blots, antisera against purified Vtg, Lv and Pv recognized the ~210 kDa Vtg band. Catshark Pv, in contrast to teleost Pvs, had a very low serine content. The catshark Vtg cDNA sequence (vtg) appeared to contain an open-reading frame consisting of domains encoding Lv, Pv and β'-component (β'-c). A phylogenetic analysis, with a consideration of genome duplication events, placed catshark vtg into the 'vtgAB type.' It is concluded that at least a single major type of Vtg protein, which is transcribed and translated from catshark vtgAB gene, is the precursor of three egg yolk proteins (Lv, Pv and β'-c) in catshark.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blotting, Western veterinary
Chromatography veterinary
Chromatography, Gel veterinary
Cluster Analysis
Computational Biology
DNA Primers genetics
DNA, Complementary genetics
Egg Proteins chemistry
Electrophoresis, Polyacrylamide Gel veterinary
Molecular Sequence Data
Molecular Weight
Phosvitin chemistry
Phylogeny
Sequence Analysis, DNA veterinary
Vitellogenins analysis
Vitellogenins chemistry
Egg Proteins genetics
Phosvitin genetics
Sharks metabolism
Vitellogenins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1573-5168
- Volume :
- 39
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Fish physiology and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22918791
- Full Text :
- https://doi.org/10.1007/s10695-012-9706-1