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[Effect of Colquhounia root tablet on IL-2 and IFN-γ mRNA expression in rats with experimental allergic encephalomyelitis].
- Source :
-
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences [Zhejiang Da Xue Xue Bao Yi Xue Ban] 2012 Jul; Vol. 41 (4), pp. 425-9. - Publication Year :
- 2012
-
Abstract
- Objective: To investigate the effect of Colquhounia root tablet on IL-2 and IFN-γ mRNA expression in experimental allergic encephalomyelitis (EAE) of rats.<br />Methods: The allergic encephalomyelitis model was established in Wistar rats by immunization with myelin basic protein of spinal cord of guinea pig and complete Freund's adjuvant. The rats in treatment group received Colquhounia root tablet (300 mg*kg(-1), BID). The symptom of EAE was observed; pathological feature and myelin of brain and spinal cord were detected with HE stain and Loyez's stain, respectively. The expressions of IL-2 and IFN-γ mRNA were assayed by RT-PCR.<br />Results: No EAE symptoms were developed in treatment group, the expressions of IL-2 and IFN-γ mRNA were 0.345 ± 0.032 and 0.353 ± 0.023, which were significantly lower than those of model group (P<0.01). The histopathologic examinations revealed that less inflammation cells around vessels and demyelination in white matter of brain and spinal cords were observed in treatment group than in model group.<br />Conclusion: Colquhounia root tablets are effective in treatment of EAE of rats, which may be associated with inhibition of the expression of IL-2 and IFN-γ mRNA.
- Subjects :
- Animals
Brain drug effects
Brain metabolism
Disease Models, Animal
Drugs, Chinese Herbal therapeutic use
Encephalomyelitis, Autoimmune, Experimental drug therapy
Female
Guinea Pigs
Male
Rats
Rats, Wistar
Drugs, Chinese Herbal pharmacology
Encephalomyelitis, Autoimmune, Experimental metabolism
Interferon-gamma metabolism
Interleukin-2 metabolism
Tripterygium
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1008-9292
- Volume :
- 41
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 22927078
- Full Text :
- https://doi.org/10.3785/j.issn.1008-9292.2012.04.011