Back to Search
Start Over
Mitochondrial ROS-K+ channel signaling pathway regulated secretion of human pulmonary artery endothelial cells.
- Source :
-
Free radical research [Free Radic Res] 2012 Dec; Vol. 46 (12), pp. 1437-45. Date of Electronic Publication: 2012 Sep 27. - Publication Year :
- 2012
-
Abstract
- The objective was to investigate the molecular mechanism of mitochondrial reactive oxygen species (ROS) signaling regulation of pulmonary artery endothelial cell (HPAEC) secretion in the condition of oxidative stress. Acrolein (40 μM) induced HPAEC mitochondrial generation of ROS, rotenone (2 μmol/L) blocked mitochondrial respiratory chain complex I, cesium chloride (CsCl, 40 mmol/L)blocked K(+)channels, and saline (0.9 g/dl) were used as control. The generations of NOS, ET-1 and VEGF were determined with ELISA in the condition of different treatment reagents namely acrolein, acrolein plus rotenone, acrolein plus CsCl and saline. In the different reagent treatment of HPAECs, acrolein increased mitochondrial ROS, membrane potential, Kv1.5 mRNA and protein expression, intracellular calcium and the generation of NOS (determining NO production), ET-1 and VEGF, and those were reduced by rotenone. CsCl decreased the increment of membrane potential, the elevation of intracellular calcium and the upregulation of NOS, E-1 and VEGF expressions, which were induced by acrolein. The present study demonstrated that mitochondrial ROS-K(+)channel regulated HPAEC secretion of NO, ET-1 and VEGF in the condition of oxidative stress. Kv1.5 channel may be an important component of ROS-K+ channel signaling pathway, and intracellular calcium contributed to mitochondrial ROS-K(+) channel signaling modulation of HPAEC secretion.
- Subjects :
- Acrolein pharmacology
Blotting, Western
Calcium metabolism
Cell Proliferation drug effects
Cells, Cultured
Endothelin-1 genetics
Endothelin-1 metabolism
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Enzyme-Linked Immunosorbent Assay
Humans
Hydrogen Peroxide metabolism
Kv1.5 Potassium Channel genetics
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Nitric Oxide Synthase genetics
Nitric Oxide Synthase metabolism
Oxidative Stress drug effects
Pulmonary Artery cytology
Pulmonary Artery drug effects
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Rotenone pharmacology
Uncoupling Agents pharmacology
Vascular Endothelial Growth Factor A genetics
Vascular Endothelial Growth Factor A metabolism
Endothelium, Vascular metabolism
Kv1.5 Potassium Channel metabolism
Mitochondria metabolism
Pulmonary Artery metabolism
Reactive Oxygen Species metabolism
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1029-2470
- Volume :
- 46
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 22928487
- Full Text :
- https://doi.org/10.3109/10715762.2012.724532