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Subnormothermic machine perfusion for non-heart-beating donor liver grafts preservation in a Swine model: a new strategy to increase the donor pool?
- Source :
-
Transplantation proceedings [Transplant Proc] 2012 Sep; Vol. 44 (7), pp. 2026-8. - Publication Year :
- 2012
-
Abstract
- We previously reported that subnormothermic machine perfusion (sMP; 20°C) is able to improve the preservation of livers obtained from non-heart-beating donors (NHBDs) in rats. We have compared sMP and standard cold storage (CS) to preserve pig livers after 60 minutes of cardiac arrest. In the sMP group livers were perfused for 6 hours with Celsior at 20°C. In the CS group they were stored in Celsior at 4°C for 6 hours as usual. To simulate liver transplantation, both sMP- and CS-preserved livers were reperfused using a mechanical continuous perfusion system with autologus blood for 2 hours at 37°C. At 120 min after reperfusion aspartate aminotransferase levels in sMP versus CS were 499 ± 198 versus 7648 ± 2806 U/L (P < .01); lactate dehydrogenase 1685 ± 418 versus 12998 ± 3039 U/L (P < .01); and lactic acid 4.78 ± 3.02 versus 10.46 ± 1.79 mmol/L (P < .01) respectively. The sMP group showed better histopathologic results with significantly less hepatic damage. This study confirmed that sMP was able to resuscitate liver grafts from large NHBD animals.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Aspartate Aminotransferases metabolism
Disaccharides
Electrolytes
Glutamates
Glutathione
Histidine
L-Lactate Dehydrogenase metabolism
Mannitol
Myocardial Contraction
Organ Preservation Solutions
Perfusion instrumentation
Swine
Body Temperature
Liver Transplantation
Models, Animal
Perfusion methods
Tissue Donors
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2623
- Volume :
- 44
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Transplantation proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 22974898
- Full Text :
- https://doi.org/10.1016/j.transproceed.2012.06.014