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Peptide nucleic acids targeting miR-221 modulate p27Kip1 expression in breast cancer MDA-MB-231 cells.
- Source :
-
International journal of oncology [Int J Oncol] 2012 Dec; Vol. 41 (6), pp. 2119-27. Date of Electronic Publication: 2012 Sep 19. - Publication Year :
- 2012
-
Abstract
- The activity of a peptide nucleic acid (PNA) targeting cancer-associated microRNA-221 is described. PNAs against miR-221 were designed in order to bind very efficiently to the target RNA strand and to undergo efficient uptake in the cells. A polyarginine-PNA conjugate targeted against miR-221 (Rpep-PNA-a221) showed both very high affinity for RNA and efficient cellular uptake without the addition of transfection reagents. Unmodified PNA with the same sequence displayed RNA binding, but cellular uptake was very poor. Consistently, only Rpep-PNA-a221 strongly inhibited miR-221. Targeting miR-221 by PNA resulted in i) lowering of the hybridization levels of miR-221 measured by RT-qPCR, ii) upregulation of p27Kip1 gene expression, measured by RT-qPCR and western blot analysis. The major conclusion of this study is that efficient delivery of anti‑miR PNA through a suitable peptide carrier (Rpep‑PNA-a221) leads to inhibition of miR-221 activity, altering the expression of miR-221-regulated functions in breast cancer cells.
- Subjects :
- Base Sequence
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p27 metabolism
Female
Gene Expression
Gene Expression Regulation, Neoplastic
Gene Order
Humans
Peptide Nucleic Acids chemical synthesis
RNA Interference
Breast Neoplasms genetics
Breast Neoplasms metabolism
Cyclin-Dependent Kinase Inhibitor p27 genetics
MicroRNAs genetics
MicroRNAs metabolism
Peptide Nucleic Acids metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 41
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 22992757
- Full Text :
- https://doi.org/10.3892/ijo.2012.1632