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[Human enterovirus 71 (EV71) resisted type I interferon induced antiviral effect].

Authors :
Cui Y
Song J
Song QQ
Sun P
Gan X
Li GQ
Wang KX
Han J
Source :
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi] 2012 Apr; Vol. 26 (2), pp. 102-4.
Publication Year :
2012

Abstract

Objective: To investigate human enterovirus 71 (EV71) resistance to type I interferon induced antiviral effect.<br />Methods: After type I interferons (alpha, beta) were incubated with HeLa cells, recombinant type I herpes simple virus (HSV-1) with green fluorescent protein (GFP) was inoculated onto the HeLa cells. HSV-1 proliferation was observed by GFP expression and PCR. After EV71 was inoculated onto HeLa cells incubated with the same quantity of interferon, proliferation of EV71 were detected by RT-PCR of 2A gene.<br />Results: Recombinant HSV-1 GFP expression and viral DNA replication obviously decreased in HeLa cells incubated with type I interferon (alpha, beta). However, EV71 effectively proliferated in the interferon irritated HeLa cell by RT-PCR.<br />Conclusion: HeLa cell irritated by type I interferon (alpha, beta) produced antiviral substance that inhibits HSV-1 proliferation. EV71 resisted the antiviral substance induced by type I interferon and could significantly replicate in the HeLa cells.

Details

Language :
Chinese
ISSN :
1003-9279
Volume :
26
Issue :
2
Database :
MEDLINE
Journal :
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
Publication Type :
Academic Journal
Accession number :
23002545