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Transcriptomic analysis of peritoneal cells in a mouse model of sepsis: confirmatory and novel results in early and late sepsis.
- Source :
-
BMC genomics [BMC Genomics] 2012 Sep 25; Vol. 13, pp. 509. Date of Electronic Publication: 2012 Sep 25. - Publication Year :
- 2012
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Abstract
- Background: The events leading to sepsis start with an invasive infection of a primary organ of the body followed by an overwhelming systemic response. Intra-abdominal infections are the second most common cause of sepsis. Peritoneal fluid is the primary site of infection in these cases. A microarray-based approach was used to study the temporal changes in cells from the peritoneal cavity of septic mice and to identify potential biomarkers and therapeutic targets for this subset of sepsis patients.<br />Results: We conducted microarray analysis of the peritoneal cells of mice infected with a non-pathogenic strain of Escherichia coli. Differentially expressed genes were identified at two early (1 h, 2 h) and one late time point (18 h). A multiplexed bead array analysis was used to confirm protein expression for several cytokines which showed differential expression at different time points based on the microarray data. Gene Ontology based hypothesis testing identified a positive bias of differentially expressed genes associated with cellular development and cell death at 2 h and 18 h respectively. Most differentially expressed genes common to all 3 time points had an immune response related function, consistent with the observation that a few bacteria are still present at 18 h.<br />Conclusions: Transcriptional regulators like PLAGL2, EBF1, TCF7, KLF10 and SBNO2, previously not described in sepsis, are differentially expressed at early and late time points. Expression pattern for key biomarkers in this study is similar to that reported in human sepsis, indicating the suitability of this model for future studies of sepsis, and the observed differences in gene expression suggest species differences or differences in the response of blood leukocytes and peritoneal leukocytes.
- Subjects :
- Animals
Cells, Cultured
DNA-Binding Proteins biosynthesis
DNA-Binding Proteins genetics
Disease Models, Animal
Early Growth Response Transcription Factors biosynthesis
Early Growth Response Transcription Factors genetics
Escherichia coli
Escherichia coli Infections microbiology
Female
Gene Expression
Gene Expression Profiling
Gene Expression Regulation
Genetic Markers
Hepatocyte Nuclear Factor 1-alpha
Kruppel-Like Transcription Factors biosynthesis
Kruppel-Like Transcription Factors genetics
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Oligonucleotide Array Sequence Analysis
RNA-Binding Proteins biosynthesis
RNA-Binding Proteins genetics
Repressor Proteins biosynthesis
Repressor Proteins genetics
T Cell Transcription Factor 1 biosynthesis
T Cell Transcription Factor 1 genetics
Trans-Activators biosynthesis
Trans-Activators genetics
Transcription Factors biosynthesis
Transcription Factors genetics
Transcription, Genetic
Transcriptome
Intraabdominal Infections genetics
Intraabdominal Infections microbiology
Peritoneum microbiology
Sepsis genetics
Sepsis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2164
- Volume :
- 13
- Database :
- MEDLINE
- Journal :
- BMC genomics
- Publication Type :
- Academic Journal
- Accession number :
- 23009705
- Full Text :
- https://doi.org/10.1186/1471-2164-13-509