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[Effect of artemisinin on the expressions of GRalpha mRNA, GRbeta mRNA and P300/CBP protein in lupus nephritis mice].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2012 Apr; Vol. 35 (4), pp. 608-12. - Publication Year :
- 2012
-
Abstract
- Objective: To investigate the effects of Artemisinin on the expressions of GRalpha mRNA, GRbeta mRNA and P300/CBP protein in lupus nephritis mice.<br />Methods: Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table: control group, model group, prednisone group administrated with 6.45 mg/kg Artemisinin (Art) suspension. A mice model of LN was established by injection with living lymph cell suspension. The expressions of GC receptor alpha (GRalpha) mRNA, GC receptor beta (GRbeta) mRNA in peripheral blood mononuclear cells (PBMCs), and transcriptional coactivator P300/CBP protein in renal tissue were respectively measured by the technique of RT - PCR and immunohistochemical assay.<br />Results: Compared with the model group. The expression of transcriptional coactivator P300/CBP protein in renal tissue and GRa mRNA in PBMCs of treatment groups was increased significantly, GRbeta mRNA expression was significantly decreased (P < 0.05 or P < 0.01). And the Art group had a better effect on the expression of GRalpha mRNA and transcriptional coactivator P300/CBP protein than that of the prednisone group (P < 0.01).<br />Conclusion: The underlying therapeutic mechanism may be correlated with the regulation of Art on the expressions of GRalpha mRNA, GRbeta mRNA in PBMC and transcriptional coactivator P300/CBP protein in renal tissue.
- Subjects :
- Animals
Artemisia annua chemistry
Artemisinins administration & dosage
Disease Models, Animal
Female
Leukocytes, Mononuclear drug effects
Lupus Nephritis drug therapy
Mice
Mice, Inbred Strains
Prednisone administration & dosage
Prednisone pharmacology
RNA, Messenger genetics
RNA, Messenger metabolism
Receptors, Glucocorticoid genetics
Reverse Transcriptase Polymerase Chain Reaction
p300-CBP Transcription Factors genetics
Artemisinins pharmacology
Leukocytes, Mononuclear metabolism
Lupus Nephritis metabolism
Receptors, Glucocorticoid metabolism
p300-CBP Transcription Factors metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 35
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 23019910