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Modification of plant Rac/Rop GTPase signalling using bacterial toxin transgenes.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2013 Jan; Vol. 73 (2), pp. 314-24. Date of Electronic Publication: 2012 Nov 08. - Publication Year :
- 2013
-
Abstract
- Bacterial protein toxins which modify Rho GTPase are useful for the analysis of Rho signalling in animal cells, but these toxins cannot be taken up by plant cells. We demonstrate in vitro deamidation of Arabidopsis Rop4 by Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1) and glucosylation by Clostridium difficile toxin B. Expression of the catalytic domain of CNF1 caused modification and activation of co-expressed Arabidopsis Rop4 GTPase in tobacco leaves, resulting in hypersensitive-like cell death. By contrast, the catalytic domain of toxin B modified and inactivated co-expressed constitutively active Rop4, blocking the hypersensitive response caused by over-expression of active Rops. In transgenic Arabidopsis, both CNF1 and toxin B inhibited Rop-dependent polar morphogenesis of leaf epidermal cells. Toxin B expression also inhibited Rop-dependent morphogenesis of root hairs and trichome branching, and resulted in root meristem enlargement and dwarf growth. Our results show that CNF1 and toxin B transgenes are effective tools in Rop GTPase signalling studies.<br /> (© 2012 The Authors The Plant Journal © 2012 Blackwell Publishing Ltd.)
- Subjects :
- Amino Acid Sequence
Arabidopsis genetics
Arabidopsis Proteins genetics
Bacterial Toxins genetics
Escherichia coli metabolism
GTP-Binding Proteins genetics
Molecular Sequence Data
Plant Epidermis cytology
Plant Epidermis metabolism
Plant Leaves cytology
Plant Leaves metabolism
Plant Roots growth & development
Plant Roots metabolism
Plants, Genetically Modified
Nicotiana genetics
Nicotiana metabolism
rac GTP-Binding Proteins genetics
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Bacterial Toxins metabolism
GTP-Binding Proteins metabolism
rac GTP-Binding Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 73
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 23020817
- Full Text :
- https://doi.org/10.1111/tpj.12040