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Phycocyanin may suppress D-galactose-induced human lens epithelial cell apoptosis through mitochondrial and unfolded protein response pathways.
- Source :
-
Toxicology letters [Toxicol Lett] 2012 Nov 23; Vol. 215 (1), pp. 25-30. Date of Electronic Publication: 2012 Oct 02. - Publication Year :
- 2012
-
Abstract
- Apoptosis of lens epithelial cell (LEC) plays an important role in cataract formation, and its prevention may be one of the therapeutic strategies in treating cataract. This study used human lens epithelial cell (hLEC) line SRA01/04 to investigate the protective effect and mechanism of phycocyanin on glactose-induced apoptosis in hLEC. hLECs were cultured in D/F(12)-10% FBS medium containing 125mM d-galactose with or without phycocyanin. Cell viability was assessed by methylthiazol tetrazolium (MTT) assay. Cell apoptosis was elevated with Wright-Giemsa staining, AO/EB double staining, and DNA fragmentation assay. Mitochondrial apoptosis-associated molecules and unfolded protein response-associated molecules from cultured SRA01/04 cells were quantified using protein blot analysis. The results demonstrated that phycocyanin suppressed SRA01/04 cells' morphologic changes and apoptosis induced by d-galactose, inhibited the expression and activation of caspase 3, alternated the Bax/Bcl-2 ratio, and down-regulated the level of p53, GRP78, and CHOP in d-galactose-treated SRA01/04 cells. These results suggest that phycocyanin might suppress d-galactose-induced hLEC apoptosis through two pathways: mitochondrial pathway, involving p53 and Bcl-2 family protein expression, and unfolded protein response pathway, involving GRP78 and CHOP expression.<br /> (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Apoptosis Regulatory Proteins metabolism
Blotting, Western
Caspase 3 metabolism
Cell Survival drug effects
Cells, Cultured
DNA Fragmentation drug effects
Endoplasmic Reticulum Chaperone BiP
Enzyme Activation drug effects
Epithelial Cells ultrastructure
Humans
Lens, Crystalline drug effects
Microscopy, Fluorescence
Tetrazolium Salts
Thiazoles
Apoptosis drug effects
Epithelial Cells drug effects
Galactose antagonists & inhibitors
Galactose toxicity
Lens, Crystalline cytology
Mitochondria drug effects
Phycocyanin pharmacology
Unfolded Protein Response drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 215
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 23036742
- Full Text :
- https://doi.org/10.1016/j.toxlet.2012.09.017