Methods for measuring DNA adducts and abasic sites II: methods for measurement of DNA adducts.

Authors :: Swenberg JA
Ham AJ
Koc H
La DK
Morinello EJ
Pachkowski BF
Ranasinghe A
Upton PB
Source :: Current protocols in toxicology [Curr Protoc Toxicol] 2002 Aug; Vol. Chapter 3, pp. Unit3.9.
Publication Year :: 2002
Abstract: This unit contains protocols for analyzing DNA adducts separated from the DNA backbone. HPLC is used to quantify total guanine or ribo- or deoxynucleotides as well as methods for analyzing specific adducts. These methods include HPLC with electrochemical detection, immunoaffininty chromatography to enrich for specific adducts, and gas and liquid chromatography in combination with HPLC and mass spectrometry.

Subjects :: Cell Line
Chromatography, Affinity methods
Chromatography, High Pressure Liquid methods
Electrochemical Techniques
Gas Chromatography-Mass Spectrometry
Humans
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
DNA chemistry
DNA Adducts analysis

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