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Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex.
- Source :
-
Cytoskeleton (Hoboken, N.J.) [Cytoskeleton (Hoboken)] 2012 Dec; Vol. 69 (12), pp. 1059-68. Date of Electronic Publication: 2012 Oct 09. - Publication Year :
- 2012
-
Abstract
- The tpg1 mutant of Chlamydomonas lacks the tubulin polyglutamylase TTLL9 and is deficient in flagellar tubulin polyglutamylation. It exhibits slow swimming, whereas the double mutant with oda2 (a slow-swimming mutant that lacks outer-arm dynein) is completely nonmotile. Thus, tubulin polyglutamylation must be important for the functioning of inner-arm dynein(s). In this study, we show that the tpg1 mutation only slightly affects the motility of mutants that lack dynein "e," one of the seven species of major inner-arm dyneins, whereas it greatly reduces the motility of mutants lacking other inner-arm dynein species. This suggests that dynein e is the main target of motility regulation by tubulin polyglutamylation. Furthermore, the motility of various mutants in the background of the tpg1 mutation raises the possibility that tubulin polyglutamylation also affects the dynein regulatory complex, a dynein e-associated key regulator of flagellar motility, which possibly constitutes the interdoublet (nexin) link. Tubulin polyglutamylation thus may play a central role in the regulation of ciliary and flagellar motility. © 2012 Wiley Periodicals, Inc.<br /> (Copyright © 2012 Wiley Periodicals, Inc.)
- Subjects :
- Cell Movement physiology
Chlamydomonas enzymology
Chlamydomonas genetics
Chlamydomonas metabolism
Chlamydomonas physiology
Microtubules metabolism
Peptide Synthases deficiency
Peptide Synthases genetics
Plant Proteins genetics
Plant Proteins metabolism
Dyneins metabolism
Flagella physiology
Peptide Synthases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1949-3592
- Volume :
- 69
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cytoskeleton (Hoboken, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 23047862
- Full Text :
- https://doi.org/10.1002/cm.21075