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The Xenopus doublesex-related gene Dmrt5 is required for olfactory placode neurogenesis.
- Source :
-
Developmental biology [Dev Biol] 2013 Jan 01; Vol. 373 (1), pp. 39-52. Date of Electronic Publication: 2012 Oct 09. - Publication Year :
- 2013
-
Abstract
- The Dmrt (doublesex and mab-3 related transcription factor) genes encode a large family of evolutionarily conserved transcription factors whose function in sex specific differentiation has been well studied in all animal lineages. In vertebrates, their function is not restricted to the developing gonads. For example, Xenopus Dmrt4 is essential for neurogenesis in the olfactory system. Here we have isolated and characterized Xenopus Dmrt5 and found that it is coexpressed with Dmrt4 in the developing olfactory placodes. As Dmrt4, Dmrt5 is positively regulated in the ectoderm by neural inducers and negatively by proneural factors. Both Dmrt5 and Dmrt4 genes are also activated by the combined action of the transcription factor Otx2, broadly transcribed in the head ectoderm and of Notch signaling, activated in the anterior neural ridge. As for Dmrt4, knockdown of Dmrt5 impairs neurogenesis in the embryonic olfactory system and in neuralized animal caps. Conversely, its overexpression promotes neuronal differentiation in animal caps, a property that requires the conserved C-terminal DMA and DMB domains. We also found that the sea anenome Dmrt4/5 related gene NvDmrtb also induces neurogenesis in Xenopus animal caps and that conversely, its knockdown in Nematostella reduces elav-1 positive neurons. Together, our data identify Dmrt5 as a novel important regulator of neurogenesis whose function overlaps with that of Dmrt4 during Xenopus olfactory system development. They also suggest that Dmrt may have had a role in neurogenesis in the last common ancestor of cnidarians and bilaterians.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
COS Cells
Chlorocebus aethiops
DNA Primers genetics
DNA, Complementary genetics
Electrophoretic Mobility Shift Assay
Gene Knockdown Techniques
In Situ Nick-End Labeling
Otx Transcription Factors metabolism
Plasmids genetics
Reverse Transcriptase Polymerase Chain Reaction
Sea Anemones genetics
Species Specificity
Transcription Factors genetics
Transcription Factors physiology
Xenopus genetics
Xenopus Proteins genetics
Xenopus Proteins physiology
Neurogenesis physiology
Olfactory Mucosa embryology
Transcription Factors metabolism
Xenopus embryology
Xenopus Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-564X
- Volume :
- 373
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 23064029
- Full Text :
- https://doi.org/10.1016/j.ydbio.2012.10.003