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Chronic hepatitis E virus infection is specifically associated with an interferon-related transcriptional program.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2013 Jan 01; Vol. 207 (1), pp. 125-32. Date of Electronic Publication: 2012 Oct 16. - Publication Year :
- 2013
-
Abstract
- Background: Hepatitis E virus (HEV) is a new causative agent of chronic hepatitis in solid organ transplant recipients. Clinical studies suggest that the occurrence and persistence of chronic HEV infection are related to the immunological status of patients.<br />Methods: We used whole-genome microarray and quantitative reverse transcription polymerase chain reaction (qRT-PCR) to compare the transcriptional profiles of whole blood from 8 kidney transplant recipients with chronic HEV infection and 8 matched kidney transplant recipients without HEV infection.<br />Results: We found that 30 genes in HEV-infected patients were upregulated, compared with those in control patients, as determined by microarray analysis. In contrast, no genes were downregulated. The 30 upregulated genes included 25 interferon-stimulated genes. Increased expression of the genes that encode IFIT1, IFI44L, RSAD2, EPSTI1, and ISG15 was confirmed by qRT-PCR. Interestingly, the expression levels of these genes were associated with the persistence of HEV infection.<br />Conclusions: Increased expression of interferon-stimulated genes may favor the persistence of an HEV infection. Whether the expression of interferon-stimulated genes is a marker of ongoing viremia or independent prognostic marker of HEV clearance needs further investigations.<br />Clinical Trials Registration: NCT01090232.
- Subjects :
- Adult
Aged
Case-Control Studies
Female
France epidemiology
Gene Expression Profiling
Gene Expression Regulation, Viral
Hepatitis Antibodies blood
Hepatitis E epidemiology
Hepatitis E genetics
Hepatitis E virology
Hepatitis E virus genetics
Hepatitis, Chronic epidemiology
Hepatitis, Chronic genetics
Hepatitis, Chronic virology
Humans
Interferons immunology
Kidney Transplantation immunology
Male
Middle Aged
Oligonucleotide Array Sequence Analysis
Postoperative Complications immunology
Postoperative Complications virology
Prospective Studies
RNA, Viral blood
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Hepatitis E immunology
Hepatitis E virus immunology
Hepatitis, Chronic immunology
Interferons genetics
Kidney Transplantation adverse effects
Postoperative Complications epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 207
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 23072754
- Full Text :
- https://doi.org/10.1093/infdis/jis632