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Simultaneous detection of duplex DNA oligonucleotides using a SERS-based micro-network gradient chip.
- Source :
-
Lab on a chip [Lab Chip] 2012 Dec 21; Vol. 12 (24), pp. 5160-7. - Publication Year :
- 2012
-
Abstract
- We report the development of a programmable surface-enhanced Raman scattering (SERS)-based micro-network gradient platform to simultaneously detect two different types of DNA oligomer mixtures. The utility of this platform was demonstrated by quantitative analysis of two breast cancer-related (BRCA1) DNA oligomer mixtures. To generate on-demand concentration gradients, the microfluidic circuit was designed using an electric-hydraulic analogy. Then a multi-gradient microfluidic channel was fabricated based on the theoretical design of the concentration control module. These micro-network structures automatically produce a series of different concentration gradients by continuously mixing Cy3-labeled DNA oligomers (BRAC1-Mutation) with TAMRA-labeled DNA oligomer (BRAC1-Wild). The SERS signals for different ratios of duplex DNA oligomer mixtures, adsorbed on the surface of silver nanoparticles, were measured under flowing conditions. Total analysis time from serial mixing to SERS detection takes less than 10 min because all experimental conditions are automatically controlled inside the exquisitely designed microfluidic channel. This novel SERS-based DNA sensing technology in a micro-network gradient channel is expected to be a powerful analytical tool to simultaneously detect multiple DNA oligomer mixtures.
- Subjects :
- BRCA1 Protein genetics
Base Sequence
DNA genetics
Mutation
Oligodeoxyribonucleotides genetics
Surface Properties
Time Factors
DNA analysis
DNA chemistry
Microfluidic Analytical Techniques instrumentation
Oligodeoxyribonucleotides analysis
Oligodeoxyribonucleotides chemistry
Spectrum Analysis, Raman instrumentation
Subjects
Details
- Language :
- English
- ISSN :
- 1473-0189
- Volume :
- 12
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Lab on a chip
- Publication Type :
- Academic Journal
- Accession number :
- 23081724
- Full Text :
- https://doi.org/10.1039/c2lc40890b