β2-Adrenergic receptor solutions for structural biology analyzed with microscale NMR diffusion measurements.

Authors :: Horst R
Stanczak P
Stevens RC
Wüthrich K
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2013 Jan 02; Vol. 52 (1), pp. 331-5. Date of Electronic Publication: 2012 Oct 24.
Publication Year :: 2013
Abstract: Microcoil NMR measurements were performed to determine the final composition of solutions of the β(2)-adrenergic receptor (β(2)AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β(2)AR. Standards are established for the reproducible preparation of G-protein-coupled receptor solutions for crystallization trials and solution NMR studies.<br /> (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Diffusion
Humans
Micelles
Nuclear Magnetic Resonance, Biomolecular methods
Solutions
Receptors, Adrenergic, beta-2 chemistry

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