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Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 2013 Jul; Vol. 1833 (7), pp. 1641-56. Date of Electronic Publication: 2012 Nov 08. - Publication Year :
- 2013
-
Abstract
- Preferential loading of the complementary bioluminescent (f-aequorin) and fluorescent (Calcium Green-1 dextran) Ca(2+) reporters into the yolk syncytial layer (YSL) of zebrafish embryos, revealed the generation of stochastic patterns of fast, short-range, and slow, long-range Ca(2+) waves that propagate exclusively through the external YSL (E-YSL). Starting abruptly just after doming (~4.5h post-fertilization: hpf), and ending at the shield stage (~6.0hpf) these distinct classes of waves propagated at mean velocities of ~50 and ~4μm/s, respectively. Although the number and pattern of these waves varied between embryos, their initiation site and arcs of propagation displayed a distinct dorsal bias, suggesting an association with the formation and maintenance of the nascent dorsal-ventral axis. Wave initiation coincided with a characteristic clustering of YSL nuclei (YSN), and their associated perinuclear ER, in the E-YSL. Furthermore, the inter-YSN distance (IND) appeared to be critical such that Ca(2+) wave propagation occurred only when this was <~8μm; an IND >~8μm was coincidental with wave termination at shield stage. Treatment with the IP3R antagonist, 2-APB, the Ca(2+) buffer, 5,5'-dibromo BAPTA, and the SERCA-pump inhibitor, thapsigargin, resulted in a significant disruption of the E-YSL Ca(2+) waves, whereas exposure to the RyR antagonists, ryanodine and dantrolene, had no significant effect. These findings led us to propose that the E-YSL Ca(2+) waves are generated mainly via Ca(2+) release from IP3Rs located in the perinuclear ER, and that the clustering of the YSN is an essential step in providing a CICR pathway required for wave propagation. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Aequorin metabolism
Animals
Calcium Channels metabolism
Cell Nucleus metabolism
Egg Yolk cytology
Embryo, Nonmammalian cytology
Endoplasmic Reticulum metabolism
Endoplasmic Reticulum ultrastructure
Inositol 1,4,5-Trisphosphate Receptors metabolism
Microscopy, Confocal
Microscopy, Fluorescence, Multiphoton
Signal Transduction
Zebrafish metabolism
Zebrafish Proteins metabolism
Blastula metabolism
Calcium Signaling physiology
Egg Yolk metabolism
Embryo, Nonmammalian metabolism
Gastrula metabolism
Zebrafish embryology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1833
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 23142640
- Full Text :
- https://doi.org/10.1016/j.bbamcr.2012.10.031