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Enantioselective induction of a glutathione-S-transferase, a glutathione transporter and an ABC transporter in maize by Metolachlor and its (S)-isomer.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (10), pp. e48085. Date of Electronic Publication: 2012 Oct 29. - Publication Year :
- 2012
-
Abstract
- The metabolism of chiral herbicides in plants remains poorly understood. Glutathione conjugation reactions are one of the principal mechanisms that plants utilize to detoxify xenobiotics. The induction by rac- and S-metolachlor of the expression of three genes, ZmGST27, ZmGT1 and ZmMRP1, encoding respectively a glutathione-S-transferase, a glutathione transporter and an ATP-binding cassette (ABC) transporter was studied in maize. The results demonstrate that the inducing effect of rac- and S-metolachlor on the expression of ZmGST27 and ZmGT1 is comparable. However, the inducing effect of rac-metolachlor on ZmMRP1 expression is more pronounced than that of S-metolachlor. Furthermore, vanadate, an ABC transporter inhibitor, could greatly reduce the difference in herbicidal activity between rac- and S-metolachlor. These results suggest that the ABC transporters may preferentially transport conjugates of rac-metolachlor, leading to a faster metabolism of the latter. Through comparing the expression of ZmGST27, ZmMRP1 and ZmGT1 after treatment by rac- and S-metolachlor, we provide novel insights into the metabolic processes of chiral herbicides in plants.
- Subjects :
- Acetamides chemistry
Gene Expression Regulation, Developmental drug effects
Gene Expression Regulation, Plant drug effects
Herbicides chemistry
Herbicides pharmacology
Plant Leaves genetics
Plant Leaves growth & development
Reverse Transcriptase Polymerase Chain Reaction
Stereoisomerism
Zea mays growth & development
ATP-Binding Cassette Transporters genetics
Acetamides pharmacology
Glutathione Transferase genetics
Membrane Transport Proteins genetics
Plant Proteins genetics
Zea mays genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23144728
- Full Text :
- https://doi.org/10.1371/journal.pone.0048085